MSH2 antibody | knockout validation | Invitrogen 33-7900

This is a knockout-validated antibody summary, based on the publication "Genetic diversity of tumors with mismatch repair deficiency influences anti-PD-1 immunotherapy response", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG1, kappa

Company: Invitrogen

Antibody: MSH2

Catalog number: 33-7900

Summary: Mouse monoclonal IgG1, kappa against the C-terminal fragment of the human MSH2 protein. Reacts with human and mouse. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry, and immunoprecipitation.

Validation Method

Western blot

Sample

WT and Msh2-knockout CT26 mouse cell lines.

Figure

Western immunoblot depicting actin control and wild type MSH2 protein (99kDa) and protein level knockout in the Msh2 gene knockout CT26 colon carcinoma cell line. Please see Suppl. Figure 2a in the article [1].

Clone note

The same clone (FE11) is sold as LifeSpan Biosciences LS-C354901; Abnova MAB14123; Invitrogen 33-7900; Dako M3639; EMD Millipore MABE284.

References
  1. Mandal R, Samstein R, Lee K, Havel J, Wang H, Krishna C, et al. Genetic diversity of tumors with mismatch repair deficiency influences anti-PD-1 immunotherapy response. Science. 2019;364:485-491 pubmed publisher