This is a knockout-validated antibody summary, based on the publication "A MutSβ-Dependent Contribution of MutSα to Repeat Expansions in Fragile X Premutation Mice?", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. From Figure 4 in [1]. Western blot of lysates of testes, brain, liver and ovary of wild-type mice and mice deleted for MSH2, MSH3 or MSH6. Blots were probed with anti-MSH2 antibody. Laminin B1 and actin serve as a loading controls.

Rabbit polyclonal

Company: Abcam

Antibody: MSH2

Catalog number: ab70270

Summary: Rabbit polyclonal antibody raised against the sequence LEREQGEKII QEFLSKVKQM PFTEMSEENI TIKLKQLKAE VIAKNNSFVN EIISRIKVTT which coreesponds to residues 875-934 from the C-terminal region of human MSH2.

Supplier recommended for WB, IP, ICC and IHC.

Reacts with mouse and human MSH2.

Western blot

Lysates of testes, brain, liver and ovary of wild-type mice and mice deleted for MSH2, MSH3 or MSH6.

TBST containing 5% ECL Prime blocking agent (GE Healthcare).

Anti-MSH2 antibody diluted 1:10000.

Anti-rabbit IgG (NA934V; GE Healthcare).

ECL Prime detection reagent (GE Healthcare).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.