This is a knockout-validated antibody summary, based on the publication "RhoA, Rac1, and Cdc42 differentially regulate αSMA and collagen I expression in mesenchymal stem cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1 (kappa light chain)
Company: Santa Cruz
Antibody: MRCKα
Catalog number: sc-374568
Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 467-556 mapping within an internal region of human MRCKα. Recommended for detection of MRCKα of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.
Western blot
WT and MRCKα-KO mesenchymal stem cells.
5% milk in TBS, 0.05% Tween (TBS-T) for 60 min.
Overnight at 4 °C.
Corresponding secondary antibodies (Jackson Immunoresearch) for 60 min.
Luminata Western horseradish peroxidase chemiluminescence substrate detection reagent (Millipore) for 1 min at room temperature. Luminescence was detected with medical X-ray film (Agfa).
Representative Western blotting of the indicated proteins in mesenchymal stem cells with knockout of MRCKα. Please see Figure 7f in the article [1].
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