MPG antibody | knockout validation | LSBio LS-C133325

This is a knockout-validated antibody summary, based on the publication "Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

MPG antibody | knockout validation | LSBio LS-C133325 figure 1
Figure 1. Immunoblot of HEK293T whole-cell extracts (WCEs) from WT, AAG−/−, ELP1−/−, and AAG−/−ELP1−/− cell lines generated via CRISPR-Cas9 technology. From [1].
Antibody information

Mouse monoclonal IgG2a,k

Company: LSBio

Antibody: MPG

Catalog number: LS-C133325

Summary: Mouse monoclonal IgG2a,k against a partial recombinant MPG protein (AAG, aa 1-90). Reacts with human. Suitable for western blot, immunohistochemistry, immunohistochemistry (paraffin), immunofluorescence and ELISA.

Validation Method

Western blot

Sample

WT, AAG−/− HEK cells.

Primary incubation

1:1,000 dilution.

Secondary incubation

1:15,000 dilution IR Dye-conjugated secondary antibodies (Li-COR Biosciences, 925-32210).

Detection

The signal was visualized by using direct IR fluorescence via the Odyssey Scanner, LI-COR Biosciences.

Clone note

The same clone (1E10) is sold as LifeSpan Biosciences LS-C133325; Abnova H00004350-M04; Epigentek A-6110-100.

References
  1. Montaldo N, Bordin D, Brambilla A, Rösinger M, Fordyce Martin S, Bjørås K, et al. Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression. Nat Commun. 2019;10:5460 pubmed publisher