MPC1 antibody | knockout validation | Cell Signaling 14462

This is a knockout-validated antibody summary, based on the publication "Mitochondrial pyruvate carrier is required for optimal brown fat thermogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

MPC1 antibody | knockout validation | Cell Signaling 14462 figure 1
Figure 1. Western blot analysis of differentiated brown Mpc1F/F adipocytes expressing pMSCV2 or CreERT2 treated with ethanol or 4-hydroxy tamoxifen. N = 3. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: MPC1

Catalog number: 14462

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues near the carboxy terminus of human MPC1 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and Mpc1-KO brown adipocytes from mice.

Blocking agent

5% milk/PBST for 1 hr.

Primary incubation

1:1,000 dilution in 5% BSA/PBST overnight at 4°C.

Secondary incubation

1:4,000 dilution horseradish peroxidase-conjugated secondary antibodies for 1 hr.

Detection

WesternSure Premium Chemiluminescent substrate (Cat# C807723-02, LI-COR Biosciences) and detected by ChemiDoc MP Imaging System (BioRad).

References
  1. Panic V, Pearson S, Banks J, Tippetts T, Velasco Silva J, Lee S, et al. Mitochondrial pyruvate carrier is required for optimal brown fat thermogenesis. elife. 2020;9: pubmed publisher