This is a knockout-validated antibody summary, based on the publication "MMP-2 is localized to the mitochondria-associated membrane of the heart", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Abcam

Antibody: MMP-2

Catalog number: ab92536

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to human MMP2 aa 550-650 (C terminal). Reacts with human, mouse and rat. Suitable for western blot, immunocytochemistry/immunofluorescence and flow cytometry. Unsuitable for immunohistochemistry (paraffin).

Western blot | Immunohistochemistry

WB: Cytosolic extracts prepared from the hearts of male MMP-2 knockout (KO) mice on a C57Bl/6J background (1, 21) and C57Bl/6J wild-type (WT) mice. IHC: heart sections from MMP-2 WT and KO mice. Tissues were fixed in 0.1% glutaraldehyde, 4% paraformaldehyde, and 4% sucrose in 0.1 M sodium phosphate buffer (pH 7.4) overnight at 4°C.

IHC: 1:10,000 overnight at 4°C.

WB: horseradish peroxidase-conjugated goat anti-rabbit IgG (CLCC42007, Cedarlane).

IHC: Colloidal gold (18 nm)-conjugated, donkey anti-mouse IgG secondary antibodies were used at a 1:10 dilution for 90 min at room temperature.

ECL Prime reagent (Amersham) and autoradiography film or scanned using a charge-coupled device-based imager (Carestream 4000 MM Pro Image Station).

Please see Figure 1 and 3 in the article [1].