MLX antibody | knockout validation | Cell Signaling 85570

This is a knockout-validated antibody summary, based on the publication "The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

MLX antibody | knockout validation | Cell Signaling 85570 figure 1
Figure 1. IF analysis of WT versus MLXKO testis and epididymis stained with either secondary alone, or with anti-MLX then secondary antibody (200×, scale bar = 200 uM). From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: MLX

Catalog number: 85570

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Val286 of human MLX protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation (IP) and Chromatin IP.

Validation Method

Western blot | Immunohistochemistry

MLX antibody | knockout validation | Cell Signaling 85570 figure 2
Figure 2. WB analysis of isolated seminiferous tubule cells from WT and MLXKO mice. From [1].
Sample

Control and Mlx−/− mouse testis.

Blocking agent

WB: 5% Milk in TBST.

IHC: blocking reagent (Dako).

Primary incubation

WB: in blocking buffer.

IHC: Primary Dilution Buffer (Dako).

Secondary incubation

WB: HRP conjugated secondary antibody.

IHC: Alexa-Flour 488nm secondary antibodies.

Detection

WB: Chemiluminescent detection using Pro-Signal Blotting Film (Genesee Scientific, San Diego, CA, USA).

References
  1. Carroll P, Freie B, Cheng P, Kasinathan S, Gu H, Hedrich T, et al. The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis. PLoS Biol. 2021;19:e3001085 pubmed publisher