MLL2/KMT2B antibody | knockout validation | Cell Signaling 63735

This is a knockout-validated antibody summary, based on the publication "Therapeutic Targeting of Preleukemia Cells in a Mouse Model of NPM1 Mutant Acute Myeloid Leukemia", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: MLL2/KMT2B

Catalog number: 63735

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the carboxy terminus of human MLL2/KMT2B protein. Reacts with human. Suitable for western blot, immunoprecipitation and immunofluorescence/immunocytochemistry.

Validation Method

Western blot

Sample

WT and MLL2-KO OCI-AML3 cells.

Figure

Immunoblot validation of Cas9-mediated knock-out of MLL2 in OCI-AML3 cells 5 days after lentiviral transduction with three independent sgRNAs. Please see Figure S8a in the article [1].

References
  1. Uckelmann H, Kim S, Wong E, Hatton C, Giovinazzo H, Gadrey J, et al. Therapeutic targeting of preleukemia cells in a mouse model of NPM1 mutant acute myeloid leukemia. Science. 2020;367:586-590 pubmed publisher