This is a knockout-validated antibody summary, based on the publication "Combination of IAP antagonist and IFNγ activates novel caspase-10- and RIPK1-dependent cell death pathways", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Abcam

Antibody: MLKL (phospho S358)

Catalog number: ab187091

Summary: Rabbit monoclonal IgG against a synthetic peptide within human MLKL (aa 350-450, phospho S358). Reacts with human. Does not react with mouse and rat. Suitable for western blot, dot blot and immunohistochemistry (paraffin).

Western blot

WT and MLKL−/− HT29 cells. Cells were lysed in DISC lysis buffer on ice for 20 min before the addition of SDS sample loading buffer or lysed directly in SDS lysis buffer (126 mM Tris-HCl, pH 8, 20% (v/v) glycerol, 4% (w/v) SDS, 0.02% (w/v) bromophenol blue, 5% (v/v) 2-mercaptoethanol) boiled and sonicated.

5% milk in PBST.

Diluted in 2% BSA in PBST.

Immunoblot of wild-type, CRISPR/Cas9 MLKL−/−, RIPK3−/− and RIPK1−/− HT29 cells treated with IFNγ/SM, QVD and Nec-1 or not treated for 24 h. Please see Figure 4d in the article [1].