This is a knockout-validated antibody summary, based on the publication "Caspase-8 scaffolding function and MLKL regulate NLRP3 inflammasome activation downstream of TLR3", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblots of MLKL of macrophages derived from WT or MLKL knockout mice. From [1]

Antibody: MLKL

Catalog number: MABC604

Summary: Rat monoclonal antibody validated for use in immunocytochemistry, immunoprecipitation, and western blotting for the detection of MLKL of horse, human and mouse. Expected to react with bovine, chimpanzee, rat, and Rhesus monkey based on sequence homology.

Company: Millipore, now part of MilliporeSigma

Western blot

Macrophages derived from WT or MLKL knockout mice.

1/1,000–1/2,000 dilution

Caspase-1 might not be a good housekeeping gene to use as a loading control, but the authors concluded that it showed no changes.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.