This is a knockout-validated antibody summary, based on the publication "Aberrantly expressed HORMAD1 disrupts nuclear localization of MCM8-MCM9 complex and compromises DNA mismatch repair in cancer cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blotting analyses of MLH1 proteins in WT and MLH1-KO OVCAR5 cells and MDAH2774 cells. α-tubulin was used as loading control. From [1].

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: MLH1

Catalog number: sc-271978

Summary: Mouse monoclonal IgG1 κ specific for an epitope mapping between amino acids 737-756 near the C-terminus of human MLH1. Recommended for detection of MLH1 of mouse, rat, human and origin by western blot, immunoprecipitation, immunofluorescence and ELISA. Also reactive with additional species, including canine, bovine, porcine and avian.

Western blot

WT and MLH1-KO OVCAR5 cells and MDAH2774 cells.