MLH-1 antibody | knockout validation | BD Pharmingen 551091

This is a knockout-validated antibody summary, based on the publication "Mismatch repair-signature mutations activate gene enhancers across human colorectal cancer epigenomes", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

MLH-1 antibody | knockout validation | BD Pharmingen 551091 figure 1
Figure 1. Western blot analysis of MLH1 in MLH1 wildtype and mutant clones. Beta-actin is shown as a loading control. From [1].
Antibody information

Mouse monoclonal IgG

Company: BD Pharmingen

Antibody: MLH-1

Catalog number: 551091

Summary: Mouse monoclonal IgG against full-length human recombinant MLH. Reacts with human by western blot, immunohistochemistry (paraffin and frozen).

Validation Method

Western blot

Sample

Wildtype and MLH1 KO Colo-205 cells.

Primary incubation

1:250 dilution.

Clone note

The same clone (G168-15) is sold as Santa Cruz Biotechnology sc-56161; LifeSpan Biosciences LS-C95607; Abcam ab14206; Invitrogen MA1-25669; BD Biosciences 550838, 551092, 551091.

References
  1. Hung S, Saiakhova A, Faber Z, Bartels C, Neu D, Bayles I, et al. Mismatch repair-signature mutations activate gene enhancers across human colorectal cancer epigenomes. elife. 2019;8: pubmed publisher