MINA53 antibody | knockout validation | Invitrogen 39-7300

This is a knockout-validated antibody summary, based on the publication "Environmentally-induced mdig contributes to the severity of COVID-19 through fostering expression of SARS-CoV-2 receptor NRPs and glycan metabolism", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

MINA53 antibody | knockout validation | Invitrogen 39-7300 figure 1
Figure 1. Western blotting of the indicated proteins in MDIG KO BEAS-2B cells. From [1].
Antibody information

Mouse monoclonal IgG2a, kappa

Company: Invitrogen

Antibody: MINA53

Catalog number: 39-7300

Summary: Mouse monoclonal antibody against recombinant full-length human Mina53 (MDIG). Reacts with human by western blot, immunohistochemistry, immunocytochemistry (immunofluorescence), ELISA and Chromatin IP.

Validation Method

Western blot

Sample

WT and MDIG-KO BEAS-2B cells.

Blocking agent

5% nonfat dry milk.

Primary incubation

At 4 °C overnight.

Secondary incubation

Horseradish peroxidase-coupled goat anti-mouse IgG at room temperature for 1h.

Detection

Chemiluminescent Substrate (Thermo Fisher Scientific).

References
  1. Zhang Q, Wadgaonkar P, Xu L, Thakur C, Fu Y, Bi Z, et al. Environmentally-induced mdig contributes to the severity of COVID-19 through fostering expression of SARS-CoV-2 receptor NRPs and glycan metabolism. Theranostics. 2021;11:7970-7983 pubmed publisher