This is a knockout-validated antibody summary, based on the publication "EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of MICU2 in WT, MICU2-KO and MICU2-KO + rescue HEK-293T cells. α-actin is used as loading control. From [1].

Rabbit polyclonal

Company: Abcam

Antibody: MICU2

Catalog number: ab101465

Summary: Rabbit polyclonal raised against a recombinant fragment corresponding to a region within amino acids 95-238 of human EFHA1. Reacts with mouse and human protein and it is suitable for western blot.

Western blot

WT, MICU2-KO and MICU2-KO + rescue HEK-293T cells. Cells were lysed with 1x RIPA lysis buffer (Millipore) supplemented with protease inhibitors (Complete, Roche).

5% fat-free milk 1 hr at RT.

1:500 dilution overnight at 4 °C.

anti-rabbit IgG-HRP (7074S, Cell Signaling; 1:1,000) for 2 hr at RT.

ECL-plus reagent (Pierce).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.