This is a knockout-validated antibody summary, based on the publication "MICU1 regulation of mitochondrial Ca(2+) uptake dictates survival and tissue regeneration", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblots of MICU1 and Hsp70 (loading control) in MICU1+/+ and MICU1−/− MEFs lysates, from figure 1 [1].

Antibody: MICU1

Catalog number: HPA037480

Summary: Rabbit polyclonal against peptide sequence (LKGKLTIKNFLEFQRKLQHDVLKLEFERHDPVDGRITERQFGGMLLAYSGVQSKKLTAMQRQLKKHFKEGKGLTFQEVENFFTFL) of human MICU1 protein. It reacts with human protein in immunohistochemistry (formalin-fixed, paraffin-embedded sections) and western blot.

Company: Sigma

Western blot

MICU1−/− MEFs lysates prepared in RIPA buffer supplemented with proteases inhibitors (leupetin, antipain, pepstatin, phenylmethyl sulphonyl fluoride, Sigma).

1:400 overnight incubation

Membranes were scanned on the Odyssey scanner (Licor).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.