This is a knockout-validated antibody summary, based on the publication "Characterization of tools to detect and enrich human and mouse O-GlcNAcase", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: MGEA5
Catalog number: ab124807
Summary: Rabbit monoclonal IgG against a synthetic peptide within the first 50 amino acids of the N-terminus of MGEA5 (OGA). Reacts with mouse and human. Suitable for western blot, immunoprecipitation, and immunohistochemistry (paraffin). Unsuitable for immunocytochemistry or flow cytometry.
Western blot
Wild type and OGA-null MEFs.
3% (w/v) nonfat milk in TBS-T (0.05% (v/v) TWEEN-20 for 1 h at 25°C.
16–20 h at 4°C.
Anti-rabbit IgG-HRP (NA934V, GE Healthcare) for 1 h at 25°C.
Immobilon Western Chemiluminescent Substrate (EMD Millipore) and captured on autoradiography film or the Amersham Imager 600 RGB (GE Healthcare).
Western blot of OGA protein in OGT WT and null (Δ) mouse embryonic fibroblasts (MEFs), OGA wild type (WT) and null MEFs, mouse liver tissue, U2OS human osteosarcoma cells and HEPG2 human hepatocellular carcinoma cells. Please see Figure 1b in the article [1].
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