MGAT1 antibody | knockout validation | Abcam ab180578

This is a knockout-validated antibody summary, based on the publication "N-Glycans and sulfated glycosaminoglycans contribute to the action of diverse Tc toxins on mammalian cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

MGAT1 antibody | knockout validation | Abcam ab180578 figure 1
Figure 1. The efficiency of MGAT1 gene knockout was determined by western blot analysis with the indicated antibodies. GAPDH served as the loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: MGAT1

Catalog number: ab180578

Summary: Rabbit monoclonal antibody against a synthetic peptide of MGAT1. Reacts with human by western blot.

Validation Method

Western blot

Sample

Control and MGAT1-KO HeLa cells.

Secondary incubation

Corresponding secondary HRP antibodies.

Detection

Enhanced chemiluminescent detection reagent (Thermo Fisher Scientific).

References
  1. Song N, Chen L, Ren X, Waterfield N, Yang J, Yang G. N-Glycans and sulfated glycosaminoglycans contribute to the action of diverse Tc toxins on mammalian cells. PLoS Pathog. 2021;17:e1009244 pubmed publisher