This is a knockout-validated antibody summary, based on the publication "Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Hamster monoclonal IgG
Company: MBL
Antibody: MFG-E8
Catalog number: D199-3
Summary: Hamster monoclonal IgG against recombinant mouse MFG-E8. Reacts with mouse MFG-E8 short and long forms on western blot and immunohistochemistry.
Immunohistochemistry
Hearts of WT or MFG-E8 KO mice. Frozen tissue sections were fixed in acetone.
1:200 dilution overnight at 4°C.
Images were captured and analyzed using an epifluorescence microscope (BZ-9000; Keyence) or a confocal microscope (A1Rsi; Nikon).
Left ventricle sections from infarcted MFG-E8 KO mice (CD45.2+) transplanted with the bone marrow cells of WT mice (CD45.1+ ) were stained with anti-CD45.1 (green) and anti–MFG-E8 (red) antibodies (n = 3). Scale bar: 50 μm. Please see Figure 7a in the article [1].
The same clone (18A2-G10) is sold as LifeSpan Biosciences LS-C179452; MBL International D199-3.
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