This is a knockout-validated antibody summary, based on the publication "CRISPR/Cas9 mutagenesis invalidates a putative cancer dependency targeted in on-going clinical trials", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of MELK-KO clones using an antibody that recognizes a region in the N-terminal kinase domain. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: MELK

Catalog number: ab108529

Summary: Rabbit monoclonal IgG against a synthetic peptide within human MELK (N terminal). Reacts with human by western blot. Unsuitable for immunoprecipitation, immunocytochemistry, immunohistochemistry (paraffin) or flow cytometry.

Western blot

Cells were lysed with RIPA buffer (25 mM Tris, pH 7.4, 150 mM NaCl, 1% Triton X 100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, protease inhibitor cocktail (Roche, Indianapolis, Indiana), phosphatase inhibitor cocktail (Roche)).

5% non-fat milk-TBST.

1:3,000 dilution overnight at 4°C.

1:50,000 dilution anti-rabbit secondary (Abcam; ab6721).