MEKK3 antibody | knockout validation | R &D Systems MAB6095
DOI
//dx.doi.org/10.13070/ko.en.7.2247
Date
2017-04-08

This is a knockout-validated antibody summary, based on the publication "Structure and vascular function of MEKK3-cerebral cavernous malformations 2 complex", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

MEKK3 antibody | knockout validation | R &D Systems MAB6095 figure 1
Figure 1. Immunoblots of MEKK3 in freshly isolated mouse brain microvessel endothelial cells (BMECs) from three Mekk3 normal control littermates (Mekk3NCL) and three induced endothelial cells-specific Mekk3-null (Mekk3 iEC−/−) littermate pups as indicated. GAPDH expression is shown as loading control. From [1].
Antibody information

Mouse monoclonal IgG1

Company: R &D Systems

Antibody: MEKK3

Catalog number: MAB6095

Summary: Mouse monoclonal IgG1 against human MEKK3. Reacts with human by western blot and ELISA.

Validation Method

Western blot

Sample

Brain microvessel endothelial cells (BMECs) from control and induced endothelial cell-specific Mekk3-null mice.

Primary incubation

1:1,000 dilution.

Secondary incubation

1:5,000 dilution HRP-linked anti-mouse IgG (#7076, Cell Signaling Technology, MA).

References
  1. Fisher O, Deng H, Liu D, Zhang Y, Wei R, Deng Y, et al. Structure and vascular function of MEKK3-cerebral cavernous malformations 2 complex. Nat Commun. 2015;6:7937 pubmed publisher