MEKK2 antibody | knockout validation

MEKK2 antibody | knockout validation | Abcam ab33918

This is a knockout-validated antibody summary, based on the publication "MEKK2 and MEKK3 suppress Hedgehog pathway-dependent medulloblastoma by inhibiting GLI1 function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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MEKK2 antibody | knockout validation | Abcam ab33918 figure 1

Figure 1. Western blot of the indicated protein in WT and MEKK2/MEKK3-DKO NIH3T3 and Daoy cells. From [1].

Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: MEKK2

Catalog number: ab33918

Summary: Rabbit monoclonal IgG against a synthetic peptide within human MEKK2 (aa 1-100, N terminal). Reacts with mouse, rat, and human. Suitable for western blot, immunohistochemistry (frozen and paraffin), immunocytochemistry/immunofluorescence, immunoprecipitation and flow cytometry.

Validation Method

Western blot

Sample

WT and MEKK2/MEKK3-DKO NIH3T3 and Daoy cells.

Detection

ChemiScope5600 (Clinx, Shanghai) with ECL substrate.

References

Lu J, Liu L, Zheng M, Li X, Wu A, Wu Q, et al. MEKK2 and MEKK3 suppress Hedgehog pathway-dependent medulloblastoma by inhibiting GLI1 function. Oncogene. 2018;37:3864-3878 pubmed publisher

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