This is a knockout-validated antibody summary, based on the publication "Genome-scale CRISPR-Cas9 knockout screening in human cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit polyclonal

Company: Cell Signaling

Antibody: MED12

Catalog number: 4529S

Summary: Rabbit polyclonal against a synthetic peptide corresponding to residues near the carboxy terminus of human MED12 protein. Reacts with human, mouse, rat, and monkey by western blot and immunoprecipitation. Predicted to react based on 100% sequence homology with hamster, bovine, pig, and horse.

Western blot

Control and MED12-KO (lentiCRISPR-infected) A375 cells. Cells were lysed in Cell Lysis Buffer (Cell Signaling 9803) with protease inhibitors (Sigma P8340).

1:1,000 dilution.

Membranes were developed by SuperSignal West Femto ECL (Thermo/Pierce) and imaged using BioRad ChemiDoc MP imaging system.

Please see Figure S10c in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.