This is a knockout-validated antibody summary, based on the publication "Mitochondrial malic enzyme 2 promotes breast cancer metastasis via stabilizing HIF-1α under hypoxia", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. 4T1 ME2 knockout effect was checked by western blot. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: ME2

Catalog number: ab139686

Summary: Rabbit monoclonal antibody against a synthetic peptide within human ME2 (aa 100-200). Reacts with mouse, rat and human. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry (intra).

Western blot

WT and ME2 knockout 4T1 cells.

5% bovine serum albumin for 1 h.

1:2,000 dilution overnight.

1:5,000 dilution HRP-conjugated secondary antibodies.

Enhanced chemiluminescence (Tanon, China).