This is a knockout-validated antibody summary, based on the publication "The MDM2-p53-pyruvate carboxylase signalling axis couples mitochondrial metabolism to glucose-stimulated insulin secretion in pancreatic β-cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of isolated pancreatic islets from WT, MDM2 knockout (KO) and MDM2/p53 double knockout (DKO) mice. Probed with anti-MDM2 antibody (middle row). Actin serves as a loading control. From [1].

Mouse monoclonal

Company: Merck Millipore

Antibody: MDM2

Catalog number: 04-1530

Summary: Mouse monoclonal antibody raised against recombinant human MDM2.

Supplier recommended for western blot, IP, ICC and IHC.

Reacts with human and mouse MDM2.

Western blot

Immunohistochemistry

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Figure 2. Immunohistochemistry shows pancreatic sections from wild-type and MDM2 -/- mice stained with anti-MDM2 antibody. From [1].

Western blot: Isolated pancreatic islets from wild-type and knockout mice.

Immunohistochemistry: Mouse pancreas fixed with 4% paraformaldehyde and paraffin embedded.

Western blot: 1:500 dilution.

Immunohistochemistry: 1:50 dilution.

Western blot: Not stated.

Immunohistochemistry: Cy5-conjugated anti-mouse IgG (1:500)(A10525; Thermo Fisher Scientific).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.