This is a knockout-validated antibody summary, based on the publication "ZNF506-dependent positive feedback loop regulates H2AX signaling after DNA damage", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of MDC1 in control and MDC1-KO HEK293 cells after being irradiated to induce DNA damage. From [1].

Mouse monoclonal IgG1κ

Company: Millipore

Antibody: MDC1

Catalog number: 05-1572

Summary: Mouse monoclonal IgG1κ against a recombinant protein corresponding to mouse MDC1 at and around the N-terminus. Reacts with mouse, human, chimpanzee and bovine. Suitable for western blot and immunocytochemistry.

Western blot

Control and MDC1-KO HEK293 cells. NETN buffer (20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1 mM EDTA, and 0.5% Nonidet P-40) containing 10 mM NaF, 50 mM β-glycerophosphate, 1 mg ml−1 pepstatin A, and 1 mg ml−1, aprotinin was used for cell lysis.

1:1,000 dilution.

1:2,000 dilution peroxidase-AffiniPure goat anti-mouse IgG light-chain-specific (Jackson Immunoresearch, catalog #115-035-174).

The same clone (P2B11) is sold as LifeSpan Biosciences LS-C150396, LS-C152962, LS-C194906, LS-C231452, LS-C231453, LS-C231454, LS-C231455, LS-C231456, LS-C231457, LS-C231458, LS-C231459, LS-C231460, LS-C231461, LS-C231462, LS-C231464, LS-C231465, LS-C231466, LS-C231467, LS-C231468; Abnova MAB11460, MAB11461, MAB11462, MAB11463, MAB11464, MAB11465, MAB11466, MAB11467, MAB11468, MAB11469, MAB11470, MAB11471, MAB11472, MAB11473, MAB11474, MAB11475, MAB11476, MAB11477; Rockland Immunochemicals 200-301-G12; StressMarq Biosciences SMC-197D; EMD Millipore 05-1572.