This is a knockout-validated antibody summary, based on publications "The RNA genome of hepatitis E virus robustly triggers antiviral interferon response", as cited below [1], "DNA hypomethylation promotes transposable element expression and activation of immune signaling in renal cell cancer" for western blot knockout validation (figure 4a) [2], and "cGAS and DDX41-STING mediated intrinsic immunity spreads intercellularly to promote neuroinflammation in SOD1 ALS model" for western blot knockout validation (figure 3a) [3]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: MDA-5
Catalog number: 5321
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Arg470 of human MDA-5. Reacts with human and mouse. Suitable for western blot and immunoprecipitation.
Western blot
WT, MDA5-/-, and RIG-I-/-/MDA5-/- MEF cells.
Anti-rabbit IRDye-conjugated antibodies (Stressgen, Victoria, BC, Canada).
The RIG-I protein level was detected in WT, MDA5-/-, RIG-I and MDA5-/- MEF cells by western blot analysis. Please see Figure S8f in the article [1].
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