This is a knockout-examined antibody summary, based on the publication "Targeted disruption of one of the importin α family members leads to female functional incompetence in delivery", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
The antibody supplier is MBL.
Importin a5 (NPI-1) /7 monoclonal
Catalogue No is D170-3
Clone name is 2D9.
Western blot analysis [1] of Importin alpha 5 in brain, heart, thymus and lung tissue lysates from importin 5 knockout mice (impa5-/-) mice using antibody from MBL showed absence of importin 5. The clone recognizes nonspecific bands.
Mice were perfused with 0.01 m NaCl/Pi under pentobarbital sodium anesthesia. Their organs were homogenized with RIPA buffer [10 mm Tris/HCl (pH 7.2), 150 mm NaCl, 0.1% SDS, 1.0% Triton X-100, 1.0% sodium deoxycholate, 5 mm EDTA, 10 μg·mL−1 each of leupeptin, pepstatin, and aprotinin, and 1 mm phenylmethanesulfonyl fluoride). These lysates were cleared with centrifugation at 20 400 g for 30 min.
A bicinchoninic acid kit (Pierce, Rockford, IL, USA).
10 or 15 μg of tissue lysate was in each lane for SDS/PAGE.
Antibody concentration is 1:500.
Horseradish peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) at 1 : 2000.
Importin a5 expression was significantly reduced in importin 5 knockout mice (impa5-/-) (see figure 1) for western blot.
The same clone (2D9) is sold as Santa Cruz Biotechnology sc-101540; LifeSpan Biosciences LS-C179427; MBL International D170-3; Sigma-Aldrich I9908.