This is a knockout-validated antibody summary, based on the publications "TAX1BP1 Restrains Virus-Induced Apoptosis by Facilitating Itch-Mediated Degradation of the Mitochondrial Adaptor MAVS", as cited below [1] and "Hepatitis C virus exploits cyclophilin A to evade PKR" for western blot knockout validation (see [2] figure 6c). Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: MAVS

Catalog number: sc-166583

Summary: Mouse monoclonal IgG2a (kappa light chain) against amino acids 1-135 mapping within an N-terminal cytoplasmic domain of human MAVS. Recommended for detection of MAVS of human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and and ELISA.

Western blot

Control and MAVS-deficient HeLa cells infected with SeV. Cells were lysed on ice for 1 h in RIPA buffer (50 mM Tris-HCl [3, 4], 150 mM NaCl, 1% Igepal CA-630, 0.25% deoxycholate, 10 mM NaF, and 10 mM Na3VO4) freshly supplemented with protease inhibitor cocktail (Roche Applied Bioscience, Indianapolis, IN).

Antibodies were diluted in SuperBlock blocking phosphate-buffered saline (PBS) buffer (Thermo Scientific, Rockford, IL).

Enhanced chemiluminescence (ECL) reagents (Perkin-Elmer).

Western blot of the indicated proteins in TAX1BP1- and/or MAVS-deficient HeLa cells infected with SeV (25 HA units/ml) for 24 h. Please see Figure 5a in the article [1].