MAP1S antibody | knockout validation | Precision Antibody AG10006
DOI
//dx.doi.org/10.13070/ko.en.7.2258
Date
2017-04-08

This is a knockout-validated antibody summary, based on the publication "Transforming Growth Factor TGFβ Increases Levels of Microtubule-Associated Protein MAP1S and Autophagy Flux in Pancreatic Ductal Adenocarcinomas", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

MAP1S antibody | knockout validation | Precision Antibody AG10006 figure 1
Figure 1. Immunoblotting analyses MAP1S in MEF cells developed from wild-type (MAP1S+/+) or MAP1S knockout mice (MAP1S-/-). β-actin served as loading control. From [1]. In KO validation image, the antibody shows nonspecific bands, which are not observed in other images (e.g. 5E).
Antibody information

Mouse monoclonal IgG

Company: Precision Antibody

Antibody: MAP1S

Catalog number: AG10006

Summary: Mouse monoclonal IgG against recombinant MAP1S. Suitable for western blot, immunoprecipitation and ELISA.

Validation Method

Western blot

Sample

Control MAP1S+/+ or MAP1S knockout (MAP1S-/-) MEFs.

Secondary incubation

Horseradish peroxidase (HRP)-conjugated secondary antibodies (mouse #172–1011, Bio-Rad).

Detection

Enhanced chemiluminescence (ECL) Western Blotting Detection Reagents (GE Health) and X-ray films.

References
  1. Song K, Hu W, Yue F, Zou J, Li W, Chen Q, et al. Transforming Growth Factor TGFβ Increases Levels of Microtubule-Associated Protein MAP1S and Autophagy Flux in Pancreatic Ductal Adenocarcinomas. PLoS ONE. 2015;10:e0143150 pubmed publisher