This is a knockout-validated antibody summary, based on the publication "Enhancer architecture sensitizes cell specific responses to Notch gene dose via a bind and discard mechanism", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of MAML1-3 in either wild-type or MAML-deficient mK4 cells. RUNX1 is used as a loading control. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: MAML1

Catalog number: 11959

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the carboxy terminus of human MAML1 protein. Reacts with human, mouse, rat, monkey. Suitable for western blot, immunoprecipitation, and Chromatin IP.

Western blot

Wild-type or MAML-deficient mK4 cells.

5% dry milk powder in PBS with 0.1% Tween.

1:1,000 dilution overnight at 4°C.

1:5,000 dilution HRP-conjugated secondary antibodies (GE Healthcare).

SuperSignal Femto West Chemoluminescent Substrate (Thermo Fisher Scientific) and imaged with the BioRad ChemiDoc system.