This is a knockout-validated antibody summary, based on the publication "Molecular Determinants of Scaffold-induced Linear Ubiquitinylation of B Cell Lymphoma/Leukemia 10 (Bcl10) during T Cell Receptor and Oncogenic Caspase Recruitment Domain-containing Protein 11 (CARD11) Signaling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: MALT1
Catalog number: ab33921
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to human MALT1 aa 1-100 (N terminal). Reacts with human. Suitable for western blot, immunocytochemistry/immunofluorescence, and flow cytometry. Unsuitable for immunohistochemistry (paraffin).
Western blot
WT and MALT1-deficient (MALT1-KO) Jurkat cells. Cells were lysed in Den-IP lysis buffer (20 mM TrisHCl, pH 7.5, 150 mM NaCl, 30 mM NaF, 2 mM Na4P2O7,1mM EDTA, 10% glycerol, 1% Triton X-100, 5 mM N-ethylmaleimide, 1:1,000 protease inhibitor mixture (Sigma P8340)).
WT or MALT1-deficient (MALT1-KO) Jurkat T cells were stimulated with or without PMA/iono for 30 min, and lysates were resolved on SDS-PAGE, and immunoblotted with the indicated antibodies. Please see Figure 3d in the article [1].
The same clone (EP603Y) is sold as Abcam ab33921; EMD Millipore 04-580.
- Yang Y, Yang C, Chan W, Wang Z, Deibel K, Pomerantz J. Molecular Determinants of Scaffold-induced Linear Ubiquitinylation of B Cell Lymphoma/Leukemia 10 (Bcl10) during T Cell Receptor and Oncogenic Caspase Recruitment Domain-containing Protein 11 (CARD11) Signaling. J Biol Chem. 2016;291:25921-25936 pubmed
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