MAD2 antibody | knockout validation | Bethyl A300-301A
DOI
//dx.doi.org/10.13070/ko.en.6.1649
Date
2016-10-03

This is a knockout-validated antibody summary, based on the publication "The Spindle Assembly Checkpoint Is Not Essential for Viability of Human Cells with Genetically Lowered APC/C Activity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

MAD2 antibody | knockout validation | Bethyl A300-301A figure 1
Figure 1. Western blot of UBE2S, UBE2C, and MAD2 confirming the deletion of the indicated proteins in the parental ΔUBE2SΔUBE2C and the triple-knockout clone. Tubulin is used as loading control. From figure 3 [1].
Antibody information

Antibody： MAD2

Catalog number： A300-301A

Summary： Rabbit polyclonal raised against a peptide between aa. 150 and C-term of human MAD2A. Reacts with human and mouse and is predicted to react with bovine, horse, rabbit, guinea pig, and monkey. Used in western blot, immunoprecipitation and PLA.

Company： Bethyl

Validation Method

Western blot

Sample

UBE2S, UBE2C, and MAD2 triple-knockout cells

References
  1. Wild T, Larsen M, Narita T, Schou J, Nilsson J, Choudhary C. The Spindle Assembly Checkpoint Is Not Essential for Viability of Human Cells with Genetically Lowered APC/C Activity. Cell Rep. 2016;14:1829-40 pubmed publisher