Lrp4 antibody | knockout validation | UC Davis / NIH NeuroMab Facility 75-221
DOI
//dx.doi.org/10.13070/ko.en.7.1935
Date
2017-01-13

This is a knockout-validated antibody summary, based on the publication "Lrp4 in astrocytes modulates glutamatergic transmission", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal

Company: UC Davis / NIH NeuroMab Facility

Antibody: Lrp4

Catalog number: 75-221

Summary: Mouse monoclonal antibody raised against a recombinant fusion protein corresponding to residues 26-350 of the extracellular N-terminal region of mouse Lrp4.

Supplier recommend for WB and ICC.

Reacts with mouse and rat Lrp4.

Validation Method

Western blot

Sample

Hippocampal lysates from wild-type and Lrp4 knockout mice.

Blocking agent

TBS containing 5% milk powder and 0.1% Tween 20.

Primary incubation

Anti-Lrp4 antibody diluted 1:1000 in blocking buffer.

Secondary incubation

HRP-conjugated secondary antibody (ThermoFisher Scientific).

Detection

ECL reagent (Pierce / ThermoFisher Scientific).

Figure

See Figure 1 in [1].

Clone note

The same clone (N207/27) is sold as BioLegend 832201; Neuromab 73-221, 75-221.

References
  1. Sun X, Li L, Liu F, Huang Z, Bean J, Jiao H, et al. Lrp4 in astrocytes modulates glutamatergic transmission. Nat Neurosci. 2016;: pubmed publisher