Loxl3 antibody | knockout validation | Santa Cruz Biotechnology sc-377216
DOI
//dx.doi.org/10.13070/ko.en.6.1860
Date
2016-11-25

This is a knockout-validated antibody summary, based on the publication "Localized LoxL3-Dependent Fibronectin Oxidation Regulates Myofiber Stretch and Integrin-Mediated Adhesion", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal

Company: Santa Cruz Biotechnology

Antibody: Loxl3

Catalog number: sc-377216

Summary: Mouse monoclonal antibody raised against a polypeptide corresponding to residues 137-175 of human Loxl3. Supplier recommended for WB, IP, IF and elisa. Reacts with human, mouse and rat Loxl3.

Validation Method

Western blot

Sample

Lysates of mouse embryo limbs from wild-type and LOXL3 knockout animals.

Primary incubation

Anti-LOXL3 antibody diluted 1:500.

Figure

See Figure 2 in [1].

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Kraft-Sheleg O, Zaffryar-Eilot S, Genin O, Yaseen W, Soueid-Baumgarten S, Kessler O, et al. Localized LoxL3-Dependent Fibronectin Oxidation Regulates Myofiber Stretch and Integrin-Mediated Adhesion. Dev Cell. 2016;36:550-61 pubmed publisher