This is a knockout-validated antibody summary, based on the publication "Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: Lig3

Catalog number: 611876

Summary: Mouse monoclonal IgG1 against human DNA Ligase III aa. 2-115. Reacts with human, mouse, rat, and dog. Suitable for western blot. Not recommended for immunofluorescence.

Western blot

WT and Lig3−/− mouse CH12 cells.

Please see Figure 1f and S2b in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.