Lamin A/C antibody | knockout validation | Sigma-Aldrich SAB4200236

This is a knockout-validated antibody summary, based on the publication "Progerin in muscle leads to thermogenic and metabolic defects via impaired calcium homeostasis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Lamin A/C antibody | knockout validation | Sigma-Aldrich SAB4200236 figure 1
Figure 1. Western blotting for verification of lamin A/C and Sln protein expression in the soleus muscle of 30-day-old male WT, Lmna−/−, Lmna−/−Sln−/−, and Sln−/− mice. For each genotype, the expression profiles from three mice were shown. Immunoblots of β-tubulin and Gapdh are shown as loading controls. From [1].
Antibody information

Mouse monoclonal IgG2a

Company: Sigma-Aldrich

Antibody: Lamin A/C

Catalog number: SAB4200236

Summary: Mouse monoclonal IgG2a against the Ig-fold domain of human Lamin A. Reacts with human, mouse, monkey, bovine, canine, rat, and hamster. Suitable for western blot, immunoprecipitation and immunofluorescence.

Validation Method

Western blot

Sample

WT and Lmna−/− mouse muscle (soleus).

Clone note

The same clone (4C11) is sold as Enzo Life Sciences ALX-804-672-C200; MilliporeSigma SAB4200236; Cell Signaling Technology 4777, 8617.

References
  1. Wang W, Wang J, Lin W, Kao C, Hung M, Teng Y, et al. Progerin in muscle leads to thermogenic and metabolic defects via impaired calcium homeostasis. Aging Cell. 2020;19:e13090 pubmed publisher