This is a knockout-validated antibody summary, based on the publication "Activation of PDGF pathway links LMNA mutation to dilated cardiomyopathy", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1 (kappa light chain)
Company: Santa Cruz
Antibody: Lamin A/C
Catalog number: sc-376248
Summary: Mouse monoclonal IgG1 (kappa light chain) specific for an epitope mapping between amino acids 2-29 at the N-terminus of human Lamin A/C. Recommended for detection of Lamin A and Lamin C of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Also reactive with additional species, including equine, canine, bovine and porcine.
Western blot
Control and mutant induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs).
Membrane Blocking Solution (Life Technologies).
Overnight at 4 °C.
Appropriate secondary antibodies for 1 h at room temperature.
ECL Western Blotting Analysis System (GE Healthcare).
Immunoblot analysis of the levels of lamin A/C in control and mutant iPSC-CMs. The anti-LMNA (E-1) antibody was used (lot C1413). GAPDH and α-tubulin are used as loading controls. Please see Figure 3b in the article [1].
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