This is a knockout-validated antibody summary, based on the publication "LY6A/E (SCA-1) expression in the mouse testis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rat monoclonal

Company: BD Bioscience

Antibody: LY6A/E

Catalog number: 557403 (Clone D7)

Summary: Rat monoclonal antibody raised against IL-2-dependent mouse T-cell line CTL-L. Supplier recommended for flow cytometry, IHC, WB, IP and activation. Reacts with mouse LY6A/E.

Immunohistochemistry

Bouin fixed samples of testes from wild-type and Ly6a -/- mice.

5% rabbit serum in Coons buffer.

Anti-LY6A/E overnight in blocking buffer at 1:50 dilution (fluorescence) or 1:200 dilution (DAB staining).

Fluorescent readout: readout: rabbit anti-rat IgG (BA4000, Vector Labs) followed by Texas red-conjugated goat anti-rabbit.

For staining: biotinylated rabbit anti-rat IgG followed by avidin-HRP conjugate.

For fluorescent readout: VECTAshield (Vector Labs) with Leitz DMIRB fluorescent microscope and Leica TCS4D confocal laser scanning microscope.

For staining: diaminobenzine.

See figure 1 in [1]. In the article, the authors do not show data for this antibody but state that the results were the same as for anti-LY6A/E (BD Biosciences 553333) which was also used in the paper and for which they present data confirming its specificity.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.