LY6A/E antibody | knockout validation | BD Bioscience 553333
DOI
//dx.doi.org/10.13070/ko.en.6.1862
Date
2016-11-25

This is a knockout-validated antibody summary, based on the publication "LY6A/E (SCA-1) expression in the mouse testis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rat monoclonal

Company: BD Bioscience

Antibody: LY6A/E

Catalog number: 553333 (Clone E13-161.7)

Summary: Rat monoclonal antibody raised against BALB/c mouse-derived "pre-T" cell hybridoma. Supplier recommended for flow cytometry, IHC and IP. Reacts with mouse LY6A/E.

Validation Method

Immunohistochemistry

Sample

Bouin fixed samples of testes from wild-type and Ly6a -/- mice.

Blocking agent

5% rabbit serum in Coons buffer.

Primary incubation

Anti-LY6A/E overnight in blocking buffer at 1:50 dilution (fluorescence) or 1:200 dilution (DAB staining).

Secondary incubation

Fluorescent readout: readout: rabbit anti-rat IgG (BA4000, Vector Labs) followed by Texas red-conjugated goat anti-rabbit.

For staining: biotinylated rabbit anti-rat IgG followed by avidin-HRP conjugate.

Detection

For fluorescent readout: VECTAshield (Vector Labs) with Leitz DMIRB fluorescent microscope and Leica TCS4D confocal laser scanning microscope.

For staining: diaminobenzine.

Figure

See Figure 1 in [1].

Clone note

The same clone (E13-161.7) is sold as BioLegend 122501, 122502, 122504, 122505, 122506, 122507, 122508, 122511, 122512, 122513, 122514, 122515, 122516, 122517, 122518, 122519, 122520, 122523, 122524; BD Biosciences 553333.

References
  1. Van Bragt M, Ciliberti N, Stanford W, de Rooij D, van Pelt A. LY6A/E (SCA-1) expression in the mouse testis. Biol Reprod. 2005;73:634-8 pubmed