LSM12 antibody | knockout validation | Abcam ab173291

This is a knockout-validated antibody summary, based on the publication "Lsm12 is an NAADP receptor and a two-pore channel regulatory protein required for calcium mobilization from acidic organelles", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

LSM12 antibody | knockout validation | Abcam ab173291 figure 1
Figure 1. Immunoblot shows the loss of Lsm12 expression in the Lsm12-KO cell line. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: LSM12

Catalog number: ab173291

Summary: Rabbit monoclonal antibody against a recombinant fragment within human LSM12 (aa 1 to the C-terminus). Reacts with human. It is predicted to work with mouse and rat. Suitable for western blot, immunocytochemistry/immunofluorescence, immunoprecipitation and flow cytometry (intra). Unsuitable for immunohistochemisry (paraffin).

Validation Method

Western blot | Immunocytochemistry

Sample

WT and Lsm12-KO HEK293 cell lines.

Primary incubation

WB: 1:1,000 dilution.

ICC: 1:100 dilution.

References
  1. Zhang J, Guan X, Shah K, Yan J. Lsm12 is an NAADP receptor and a two-pore channel regulatory protein required for calcium mobilization from acidic organelles. Nat Commun. 2021;12:4739 pubmed publisher