This is a knockout-validated antibody summary, based on the publication "The SNF2 family ATPase LSH promotes cell-autonomous de novo DNA methylation in somatic cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of the Lsh in WT and Lsh−/− MEFs and Lsh−/− MEF cell lines expressing either wild-type or catalytically inactive LSH (K237Q) form in clonal cell lines. MRE11 was used as a loading control. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: LSH

Catalog number: sc-46665

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 1-240 mapping at the N-terminus of Lsh of human origin. Recommended for detection of Lsh of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

Nuclear extracts from WT and Lsh−/− MEFs and Lsh−/− MEF cell lines expressing either wild-type or catalytically inactive LSH (K237Q) form.

4% skimmed milk in 1 × TBS; 0.1% Tween.

Diluted in blocking solution overnight.

Secondary anti-mouse IR-800 antibodies (LiCOR Biosciences) for 1 h.

Odyssey scanner (LiCOR Biosciences).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.