This is a knockout-validated antibody summary, based on the publication "Methylation-dependent regulation of HIF-1α stability restricts retinal and tumour angiogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of LDS1 in the nuclear fraction from Lsd1+/− and Lsd1−/− MEFs. Lamin A/C was used as loading control. From [1].

Rabbit polyclonal

Company: Cell Signaling Technology

Antibody: LSD1

Catalog number: 2139

Summary: Rabbit polyclonal against a synthetic peptide corresponding to residues near the amino-terminus of human LSD1 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry, flow cytometry and ChIP.

Western blot

Nuclear fraction from Lsd1+/− and Lsd1−/− MEFs.

1:1,000 dilution in 3% BSA diluted in PBS-T.

Horseradish peroxidase-conjugated secondary antibody (Jackson).

LumiFlash Ultima Chemiluminescent substrate (Visual Protein) and LAS-4000 mini (Fuji).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.