This is a knockout-validated antibody summary, based on the publication "Detection of endogenous S1292 LRRK2 autophosphorylation in mouse tissue as a readout for kinase activity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Brain, kidney, and lung tissue from WT, Lrrk2 KO and G2019S Lrrk2 mice were fractionated and total, cytosolic, crude mitochondrial, and crude microsomal fractions and analyzed by western blot. Antibodies against PDI, MEK 1/2, and Tom20 were used to detect markers for microsomes, cytosolic, and mitochondrial fractions, respectively. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: LRRK2 (phospho S935)

Catalog number: ab133450

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to human LRRK2 (phospho S935). Reacts with mouse and human. Suitable for western blot and immunocytochemistry/immunofluorescence. Unsuitable for immunohistochemistry (paraffin).

Western blot

Brain, kidney, and lung tissue from WT, Lrrk2 KO and G2019S Lrrk2 mice.

50% TBS (20 mM Tris, 0.5 M NaCl, pH 7.5), 50% Odyssey blocking buffer (Li-Cor; 927–40,000) for 1 h.

1:2,000 dilution in 50% TBS-T (20 mM Tris, 0.5 M NaCl, pH 7.5, 0.1% Tween 20), 50% Odyssey blocking buffer at 4 °C overnight.

1:15,000 dilution IRDye® 800CW Goat anti-Rabbit IgG (LiCor: 926–32211) at RT for 1 h.

Li-Cor platform.