LRP6 antibody | knockout validation | Cell Signaling 3395

This is a knockout-validated antibody summary, based on the publication "WNT Activates the AAK1 Kinase to Promote Clathrin-Mediated Endocytosis of LRP6 and Establish a Negative Feedback Loop", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

LRP6 antibody | knockout validation | Cell Signaling 3395 figure 1
Figure 1. Western blot analysis of HEK293T WT, DVL TKO clone #4, DVL TKO clone #6, and LRP5/6 DKO cells treated with WNT3A conditioned media for 9 hr. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: LRP6

Catalog number: 3395

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to an extracellular region surrounding Met988 of human LRP6 protein. Reacts with human and mouse and it is predicted to react with rat based on 100% sequence homology. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

HEK293T WT, DVL TKO, and LRP5/6 DKO cells.

Primary incubation

1:1,000 dilution overnight at 4°C with rotation.

Secondary incubation

1:5,000 dilution (LiCOR Biosciences).

Detection

LiCOR Odyssey imager.

References
  1. Agajanian M, Walker M, Axtman A, Ruela de Sousa R, Serafin D, Rabinowitz A, et al. WNT Activates the AAK1 Kinase to Promote Clathrin-Mediated Endocytosis of LRP6 and Establish a Negative Feedback Loop. Cell Rep. 2019;26:79-93.e8 pubmed publisher