This is a knockout-validated antibody summary, based on the publication "LMO7 deficiency reveals the significance of the cuticular plate for hearing function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1 (kappa light chain)
Company: Santa Cruz
Antibody: LMO7
Catalog number: sc-376807
Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 1321-1620 mapping near the C-terminus of mouse LMO7. Recommended for detection of LMO7 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.
Immunohistochemistry
WT and Lmo7-KO mice cochlea and utricle. Tissues were fixed for 30 min in 3% paraformaldehyde.
1% bovine serum albumin, 3% normal donkey serum, and 0.2% saponin in PBS for 1 h.
overnight at 4 °C
7.5 μg/ml Alexa 488-conjugated donkey anti-mouse IgG, Invitrogen) in blocking buffer for 1–3 h.
Zeiss LSM880 and Leica confocal microscopes.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.