LMO7 antibody | knockout validation | Santa Cruz sc-376807

This is a knockout-validated antibody summary, based on the publication "LMO7 deficiency reveals the significance of the cuticular plate for hearing function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

LMO7 antibody | knockout validation | Santa Cruz sc-376807 figure 1
Figure 1. LMO7 immunolabeling in WT and Lmo7 exon17 KO mice in P4 cochlea and utricle. LMO7 is in green, and F-actin in magenta. LMO7 immunoreactivity is abolished in KO cochlear and vestibular hair cells. From [1].
Antibody information

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: LMO7

Catalog number: sc-376807

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 1321-1620 mapping near the C-terminus of mouse LMO7. Recommended for detection of LMO7 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Immunohistochemistry

Sample

WT and Lmo7-KO mice cochlea and utricle. Tissues were fixed for 30 min in 3% paraformaldehyde.

Blocking agent

1% bovine serum albumin, 3% normal donkey serum, and 0.2% saponin in PBS for 1 h.

Primary incubation

overnight at 4 °C

Secondary incubation

7.5 μg/ml Alexa 488-conjugated donkey anti-mouse IgG, Invitrogen) in blocking buffer for 1–3 h.

Detection

Zeiss LSM880 and Leica confocal microscopes.

References
  1. Du T, Dewey J, Wagner E, Cui R, Heo J, Park J, et al. LMO7 deficiency reveals the significance of the cuticular plate for hearing function. Nat Commun. 2019;10:1117 pubmed publisher