This is a knockout-validated antibody summary, based on the publication "LMO7 deficiency reveals the significance of the cuticular plate for hearing function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. LMO7 immunolabeling in WT and Lmo7 exon17 KO mice in P4 cochlea and utricle. LMO7 is in green, and F-actin in magenta. LMO7 immunoreactivity is abolished in KO cochlear and vestibular hair cells. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: LMO7

Catalog number: sc-376807

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 1321-1620 mapping near the C-terminus of mouse LMO7. Recommended for detection of LMO7 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Immunohistochemistry

WT and Lmo7-KO mice cochlea and utricle. Tissues were fixed for 30 min in 3% paraformaldehyde.

1% bovine serum albumin, 3% normal donkey serum, and 0.2% saponin in PBS for 1 h.

overnight at 4 °C

7.5 μg/ml Alexa 488-conjugated donkey anti-mouse IgG, Invitrogen) in blocking buffer for 1–3 h.

Zeiss LSM880 and Leica confocal microscopes.