This is a knockout-validated antibody summary, based on the publication "LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblot of LMAN1 in mouse brain from WT (LMAN1+/+) and LMAN1 knockout (LMAN1−/−). Courtesy from the Authors [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: LMAN1

Catalog number: ab125006

Summary: Rabbit monoclonal IgG against a synthetic peptide within human LMAN1( aa 1-100). Reacts with mouse, rat and human. Suitable for western blot, immunocytochemistry/immunofluorescence and immunohistochemistry (paraffin). Unsuitable for flow cytometry or immunoprecipitation.

Western blot

Brain from WT and LMAN1 knockout (LMAN1−/−) mice. Tissues were lysed in Co-IP buffer (50 mM Tris, pH 7.5, 150 mM NaCl, and 1% Triton X-100) supplemented with Roche complete protease inhibitor cocktail.