This is a knockout-validated antibody summary, based on the publications "The tumour suppressor LKB1 regulates myelination through mitochondrial metabolism" [1] (discussed below) and "Endothelial APLNR regulates tissue fatty acid uptake and is essential for apelin's glucose-lowering effects" [2] (see extended data figure 4b in the article). Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis showing loss of Lkb1 protein in SCs but not in DRG neurons. Actin was used as a protein loading control. Courtesy of the author. Also see Figure 1c in the article [1].

Rabbit monoclonal IgG

Company: Cell Signaling Technology

Antibody: LKB1

Catalog number: 3047S

Summary: Rabbit monoclonal IgG against LKB1 partial fusion protein. Reacts with human, mouse, rat, and monkey by western blot.

Western blot

Cultured dorsal root ganglia (DRG) neurons and purified Schwann cells (SCs). Cells were lysed in RIPA buffer.

5% dry milk in Tris-buffered saline.

1:1,000 dilution overnight at 4 °C.

Horseradish peroxidase-linked secondary antibodies (Cell Signaling Technology, Danvers, MA).

X-ray films.

LKB1 clone 5c10 (05–832; dilution 1:100; from Upstate Biotechnology, now EMD Millipore, Billerica, MA) is also KO validated (IHC), Fig S1e.