This is a knockout-validated antibody summary, based on the publication "LGR4 and LGR5 Regulate Hair Cell Differentiation in the Sensory Epithelium of the Developing Mouse Cochlea", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunohistochemistry of cochlear cryosections obtained from E14.5 homozygous Lgr4-lacZ mice and their wild type counterparts stained with anti-LGR4 and anti-SOX2, which is a marker for the pro-sensory domain. Cells in the pro-sensory domain (white arrow), spiral ganglion cells (white arrowheads), and mesenchymal cells medial to the cochlear duct (gray arrow) are pointed. In the hypomorphic homozygous Lgr4-lacZ mice, anti-LGR4 staining visibly decreased compared to the wild type controls. From [1].

Rabbit polyclonal

Company: Sigma

Antibody: LGR4

Catalog number: HPA030267

Summary: Rabbit polyclonal against LGR4 recombinant protein. Reacts with human by immunohistochemistry.

Immunohistochemistry

Cochlear cryosections obtained from E14.5 wild type and homozygous Lgr4-lacZ mice.

1% bovine serum albumin (BSA) and 0.1% triton X-100 (both from Sigma–Aldrich) in PBS for 30 min at room temperature.

1:200 dilution in 0.5% BSA in PBS at 4°C overnight.

Alexa Fluor 488 donkey anti-rabbit (A21206, Molecular Probes Life Technologies) diluted in 0.5% BSA in PBS at room temperature for 1 h.

Zeiss LSM700 Scanning Confocal Microscope.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.