This is a knockout-validated antibody summary, based on the publication "Zinc finger endonuclease targeting PSIP1 inhibits HIV-1 integration", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: LEDGF

Catalog number: 2088S

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to amino acids surrounding Lys461 of human LEDGF. Reacts with human, mouse, and rat. Suitable for western blot, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry and flow cytometry.

Western blot

Wild type, mock transfected, LEDGF/p75+/− and LEDGF/p75−/− NALM-6 cell lines. Nuclear proteins were enriched using the CelLytic NuCLEAR extraction kit (Sigma), and extracts were prepared in lysis buffer (50 mM Tris-HCl [2, 3], 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 10 mM Na β-glycerophosphate, 50 mM NaF, 5 mM Na pyrophosphate, 270 mM sucrose, and 1% Triton X-100) supplemented with protease inhibitor cocktail (Roche) and 1 mM phenylmethylsulfonyl fluoride.

Overnight at 4°C.

Horseradish peroxidase-conjugated antibody for 1 h at room temperature.

SuperSignal West Pico chemiluminescent substrate (Pierce Chemical, Rockford, IL).

Please see Figure 2f in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.