This is a knockout-validated antibody summary, based on the publication "Targeted disruption of LDLR causes hypercholesterolemia and atherosclerosis in Yucatan miniature pigs", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Representative western blot of LDLR and β-tubulin (loading control) in liver extracts from LDLR+/+, LDLR+/−, and LDLR−/− pigs. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: LDLR

Catalog number: ab215980

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to human LDL Receptor (C terminal). Reacts with mouse and human. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.

Western blot

Liver extracts from LDLR+/+, LDLR+/−, and LDLR−/− pigs. Tissues were homogenized on ice in RIPA buffer (1% NP-40, 1% Na-deoxycholate, 0.1% SDS, 0.15 M NaCl, 0.01 M NaPO4 pH 8.0, 2 mM EDTA) supplemented with protease inhibitor cocktail (Sigma), 1 mM PMSF (Sigma), and 1 mM DTT.

Horseradish peroxidase-conjugated secondary antibodies (SC-2317, Santa Cruz Biotechnology)

Chemiluminescent Detection Kit (Pierce).